We study how microtubule networks are regulated to drive different processes in cells. Our primary model system is the budding yeast, Saccharomyces cerevisiae, which affords powerful genetics that we combine with quantitative microscopy and protein biochemistry to investigate basic molecular mechanisms that regulate dynamics and transport. Our goals are to build an understanding of how the microtubule network organizes the cell, how these processes are tuned for different contexts, and how defects in network function contribute to human disease.